Anti-14-3-3 alpha + beta Rabbit pAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:Rabbit IgG

Host:Rabbit

Application:WB,IHC,ICC/IF,IP,FC

Purify method:Affinity Chromatography

Species:Human,Mouse,Rat

Gene Name:

Synonyms:14 3 3 protein beta/alpha; Brain protein 14 3 3 beta isoform; GW128; HS 1; KCIP-1; KCIP1; Protein 1054; Protein kinase C inhibitor protein 1; YWHAA; YWHAB;

Gene Synonyms:

Gene Full Name:

Gene Infomation:Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2. Reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation.

Antigen:A synthesized peptide derived from human 14-3-3 alpha + beta

Antigen Synonyms:

Clonality:Polyclonal antibody

Source:

Reaction:14-3-3 alpha + beta Antibody detects endogenous levels of 14-3-3 alpha + beta

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:WB 1:500~1:1000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:30 FC 1:30

Mole Mass:28kDa

Location:Melanosome;

Concentration:

Sequence Similarity:Contains 1 thioredoxin domain.

Gene Id:

SwissProt ID:P31946

Unigene:

Nucleotide Accession:

Tissue specificity:14-3-3 alpha + beta Antibody detects endogenous levels of 14-3-3 alpha + beta

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Background:Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.