Anti-14-3-3 epsilon Rabbit pAb
- 100ug (NP21106) Please inquiry
Antibody type:Primary antibody
Label:Unconjugated
Modification:Unmodification
Isotype:Rabbit IgG
Host:Rabbit
Application:WB,IHC,ICC/IF,FC
Purify method:Affinity Chromatography
Species:Human,Mouse,Rat
Gene Name:
Synonyms:14-3-3E; 143E; KCIP-1; MDCR; Protein kinase C inhibitor protein-1; YWHAE;
Gene Synonyms:
Gene Full Name:
Gene Infomation:Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates Lys-9 and Lys-27 of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate Lys-27 of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Compared to EZH2-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation.
Antigen:A synthesized peptide derived from human 14-3-3 epsilon
Antigen Synonyms:
Clonality:Polyclonal antibody
Source:
Reaction:14-3-3 epsilon Antibody detects endogenous levels of 14-3-3 epsilon
Form:Liquid
Tested Applications:
- Western blot (1:100 to 1:500)
- Immunofluorescence (1:50 to 1:400)
- Immunohistochemistry (1:200 to 1:500)
- Flow cytometry analysis (1:200 to 1:500)
- Enzyme-linked Immunosorbent Assay (1:100-1:5000)
Clone:
Dilution:WB 1:1000~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 FC 1:50
Mole Mass:29kDa
Location:Nucleus;
Concentration:
Sequence Similarity:Contains 1 SET domain.
Gene Id:
SwissProt ID:P62258
Unigene:
Nucleotide Accession:
Tissue specificity:14-3-3 epsilon Antibody detects endogenous levels of 14-3-3 epsilon
Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Buffer condition:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Background:Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathway. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.
Molar Function:
Western Blot:Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.