Anti-4E-BP1 Rabbit pAb
- 100ug (NP11549) Please inquiry
Antibody type:Primary antibody
Label:Unconjugated
Modification:Unmodification
Isotype:Rabbit IgG
Host:Rabbit
Application:WB,IP
Purify method:Affinity purified
Species:Human,Mouse,Rat
Gene Name:4E-BP1
Synonyms:BP-1; 4EBP1; 4E-BP1; PHAS-I; EIF4EBP1
Gene Synonyms:4E-BP1; 4EBP1; 4EBP1_HUMAN; BP 1; eIF4E binding protein 1; eIF4E-binding protein 1; Eif4ebp1; Eukaryotic translation initiation factor 4E-binding protein 1; PHAS-I; PHASI; Phosphorylated heat- and acid-stable protein regulated by insulin 1.
Gene Full Name:
Gene Infomation:
Antigen:Synthetic peptide.
Antigen Synonyms:
Clonality:Polyclonal antibody
Source:
Reaction:4E-BP1 Rabbit pAb detects endogenous levels of total 4E-BP1 protein.
Form:Liquid
Tested Applications:
- Western blot (1:100 to 1:500)
- Immunofluorescence (1:50 to 1:400)
- Immunohistochemistry (1:200 to 1:500)
- Flow cytometry analysis (1:200 to 1:500)
- Enzyme-linked Immunosorbent Assay (1:100-1:5000)
Clone:
Dilution:WB 1:1000
Mole Mass:15~20kDa
Location:Cytoplasm.
Concentration:0.928mg/ml
Sequence Similarity:Belongs to the eIF4E-binding protein family.
Gene Id:1978
SwissProt ID:Q13541
Unigene:
Nucleotide Accession:
Tissue specificity:
Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Buffer condition:Purified Rabbit polyclonal antibody in PBS(pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.
Background:4E-BP1 encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
Molar Function:
Western Blot:Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.