Anti-ALK(Phospho-Tyr1586) Rabbit pAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:

Isotype:IgG

Host:Rabbit

Application:WB,IHC

Purify method:Affinity purified

Species:Human

Gene Name:alk

Synonyms:Alk;ALK tyrosine kinase receptor;ALK Tyrosine Kinase Receptor Precursor;ALK/EML4 fusion gene, included;ALK/NPM1 fusion gene, included;ALK_HUMAN;anaplastic lymphoma kinase (Ki-1);Anaplastic lymphoma kinase;Anaplastic lymphoma kinase Ki1;Anaplastic Lymphoma Kinase p80;Anaplastic Lymphoma Kinase p80;anaplastic lymphoma receptor tyrosine kinase;CD 246;CD246;CD246 Antigen;EC 2.7.10.1;Ki 1;Ki1;mutant anaplastic lymphoma kinase;NBLST 3;NBLST3;Tcrz;TFG/ALK.

Gene Synonyms:CD246; NBLST3

Gene Full Name:

Gene Infomation:This gene encodes a receptor tyrosine kinase, which belongs to the insulin receptor superfamily. This protein comprises an extracellular domain, an hydrophobic stretch corresponding to a single pass transmembrane region, and an intracellular kinase domain. It plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. This gene has been found to be rearranged, mutated, or amplified in a series of tumours including anaplastic large cell lymphomas, neuroblastoma, and non-small cell lung cancer. The chromosomal rearrangements are the most common genetic alterations in this gene, which result in creation of multiple fusion genes in tumourigenesis, including ALK (chromosome 2)/EML4 (chromosome 2), ALK/RANBP2 (chromosome 2), ALK/ATIC (chromosome 2), ALK/TFG (chromosome 3), ALK/NPM1 (chromosome 5), ALK/SQSTM1 (chromosome 5), ALK/KIF5B (chromosome 10), ALK/CLTC (chromosome 17), ALK/TPM4 (chromosome 19), and ALK/MSN (chromosome X).[provided by RefSeq, Jan 2011]

Antigen:The antiserum was produced against synthesized phosphopeptide derived from human ALK around the phosphorylation site of tyrosine 1586 (Y-G-YP-Q-Q).

Antigen Synonyms:

Clonality:Polyclonal antibody

Source:Human

Reaction:

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:

Mole Mass:176

Location:Cell membrane; Single-pass type I membrane protein. Note: Membrane attachment was crucial for promotion of neuron-like differentiation and cell proliferation arrest through specific activation of the MAP kinase pathway.

Concentration:

Sequence Similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.Contains 1 LDL-receptor class A domain.Contains 2 MAM domains.Contains 1 protein kinase domain.

Gene Id:238

SwissProt ID:

Unigene:

Nucleotide Accession:

Tissue specificity:This antibody detects endogenous levels of total ALK protein.

Storage:

Buffer condition:10mM PBS with 0.02% sodium azide, 50% glycerol.

Background:

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.