Anti-ALKBH4 Rabbit pAb
- 100ug (NP20629) Please inquiry
Antibody type:Primary antibody
Label:Unconjugated
Modification:Unmodification
Isotype:Rabbit IgG
Host:Rabbit
Application:WB,IF
Purify method:Affinity purified
Species:Human,Mouse
Gene Name:ALKBH4
Synonyms:ABH4
Gene Synonyms:
Gene Full Name:alkB homolog 4, lysine demethylase
Gene Infomation:
Antigen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-302 of human ALKBH4 (NP_060091.1).
Antigen Synonyms:Alpha-ketoglutarate-dependent dioxygenase alkB homolog 4
Clonality:Polyclonal antibody
Source:Human
Reaction:
Form:Liquid
Tested Applications:
- Western blot (1:100 to 1:500)
- Immunofluorescence (1:50 to 1:400)
- Immunohistochemistry (1:200 to 1:500)
- Flow cytometry analysis (1:200 to 1:500)
- Enzyme-linked Immunosorbent Assay (1:100-1:5000)
Clone:
Dilution:WB1:500 -1:2000; IF1:50 -1:100(Optimal dilutions should be determined by the end user)
Mole Mass:4kDa/33kDa
Location:Cytoplasm, Nucleus
Concentration:
Sequence Similarity:
Gene Id:
SwissProt ID:Q9NXW9
Unigene:54784
Nucleotide Accession:
Tissue specificity:Widely expressed, with highest expression in pancreas, ovary and spleen.
Storage:Store at -20°C. Avoid freeze / thaw cycles.
Buffer condition:PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Background:
Molar Function:Dioxygenase that mediates demethylation of actin monomethylated at 'Lys-84' (K84me1), thereby acting as a regulator of actomyosin-processes. Demethylation of actin K84me1 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration. May be involved in transcription regulation.
Western Blot:Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.