Anti-ASPSCR1 Rabbit pAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:Rabbit IgG

Host:Rabbit

Application:WB,IHC,IF,IP

Purify method:Affinity purified

Species:Human,Mouse,Rat

Gene Name:ASPSCR1

Synonyms:TUG; ASPL; ASPS; RCC17; UBXD9; UBXN9; ASPCR1

Gene Synonyms:

Gene Full Name:ASPSCR1, UBX domain containing tether for SLC2A4

Gene Infomation:The protein encoded by this gene contains a UBX domain and interacts with glucose transporter type 4 (GLUT4). This protein is a tether, which sequesters the GLUT4 in intracellular vesicles in muscle and fat cells in the absence of insulin, and redistributes the GLUT4 to the plasma membrane within minutes of insulin stimulation. Translocation t(X;17)(p11;q25) of this gene with transcription factor TFE3 gene results in a ASPSCR1-TFE3 fusion protein in alveolar soft part sarcoma and in renal cell carcinomas. Multiple alternatively spliced transcript variants have been found. [provided by RefSeq, Oct 2011]

Antigen:Recombinant fusion protein containing a sequence corresponding to amino acids 284-553 of human ASPSCR1 (NP_076988.1).

Antigen Synonyms:Tether containing UBX domain for GLUT4

Clonality:Polyclonal antibody

Source:Human

Reaction:

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:WB1:500 -1:2000; IHC1:50 -1:200; IF1:50 -1:200; IP1:50 -1:100(Optimal dilutions should be determined by the end user)

Mole Mass:80kDa

Location:Cytoplasm, Membrane, Nucleus

Concentration:

Sequence Similarity:

Gene Id:

SwissProt ID:Q9BZE9

Unigene:79058

Nucleotide Accession:

Tissue specificity:Ubiquitous. Highly expressed in testis, heart, skeletal muscle and pancreas.

Storage:Store at -20°C. Avoid freeze / thaw cycles.

Buffer condition:PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Background:

Molar Function:Tethering protein that sequesters GLUT4-containing vesicles in the cytoplasm in the absence of insulin. Modulates the amount of GLUT4 that is available at the cell surface . Enhances VCP methylation catalyzed by VCPKMT.

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.