Anti-ATF4 Rabbit pAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:Rabbit IgG

Host:Rabbit

Application:WB,IHC,ICC/IF,IP,FC

Purify method:Affinity Chromatography

Species:Human,Mouse,Rat

Gene Name:

Synonyms:Cyclic AMP-dependent transcription factor ATF-4; CREB-2; cAMP-responsive element-binding protein 2; DNA-binding protein TAXREB67;

Gene Synonyms:

Gene Full Name:

Gene Infomation:iNOS Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions.

Antigen:A synthesized peptide derived from human ATF4

Antigen Synonyms:

Clonality:Polyclonal antibody

Source:

Reaction:ATF4 Antibody detects endogenous levels of ATF4

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:WB 1:500~1:1000 IHC 1:50~1:200 ICC 1:100~1:500 IP 1:50 FC 1:50

Mole Mass:50kDa

Location:

Concentration:

Sequence Similarity:Contains 1 flavodoxin-like domain.

Gene Id:

SwissProt ID:P18848

Unigene:

Nucleotide Accession:

Tissue specificity:ATF4 Antibody detects endogenous levels of ATF4

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Background:ATF4, an activating transcription factor/cAMP-response element-binding protein family member, functions in the PERK and eIF2α ER stress responsive pathway. ER stress represses the translation of the majority of mRNAs, but selectively stimulates the translation of certain mRNAs including that of ATF-4. Induced expression of ATF-4 increases the expression of genes critical for the recovery from ER stress.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.