Anti-ATG7 Rabbit pAb
- 100ug (NP21364) Please inquiry
Antibody type:Primary antibody
Label:Unconjugated
Modification:Unmodification
Isotype:Rabbit IgG
Host:Rabbit
Application:WB,IHC,ICC/IF,IP,FC
Purify method:Affinity Chromatography
Species:Human
Gene Name:
Synonyms:ATG7; APG7-LIKE; APG7L; DKFZp434N0735; GSA7;
Gene Synonyms:
Gene Full Name:
Gene Infomation:IRFs comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection. IRF-3 can inhibit cell growth and plays a critical role in controlling the expression of genes in the innate immune response.
Antigen:A synthesized peptide derived from human ATG7
Antigen Synonyms:
Clonality:Polyclonal antibody
Source:
Reaction:ATG7 Antibody detects endogenous levels of ATG7
Form:Liquid
Tested Applications:
- Western blot (1:100 to 1:500)
- Immunofluorescence (1:50 to 1:400)
- Immunohistochemistry (1:200 to 1:500)
- Flow cytometry analysis (1:200 to 1:500)
- Enzyme-linked Immunosorbent Assay (1:100-1:5000)
Clone:
Dilution:WB 1:10000~1:50000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:20 FC 1:20
Mole Mass:78kDa
Location:Nucleus;
Concentration:
Sequence Similarity:Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
Gene Id:
SwissProt ID:O95352
Unigene:
Nucleotide Accession:
Tissue specificity:ATG7 Antibody detects endogenous levels of ATG7
Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Buffer condition:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Background:The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10.
Molar Function:
Western Blot:Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.