Anti-ATP-Citrate Lyase (C-term) Mouse mAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:IgG2a

Host:Mouse

Application:WB,ICC,FC

Purify method:Affinity purified

Species:Human,Mouse,Monkey

Gene Name:acly

Synonyms:ACL; ATPCL; CLATP

Gene Synonyms:ACL;Acly;ACLY_HUMAN;ATP citrate (pro-S) lyase;ATP citrate lyase;ATP citrate synthase; ATP-citrate (pro-S-)-lyase;ATP-citrate synthase;ATPcitrate synthase;ATPCL;Citrate cleavage enzyme;CLATP;EC 2.3.3.8;OTTHUMP00000164773.

Gene Full Name:

Gene Infomation:

Antigen:Purified recombinant human ATP-Citrate Lyase protein fragments expressed in E.coli.

Antigen Synonyms:

Clonality:Monclonal antibody

Source:Human

Reaction:This antibody detects endogenous levels of ATP-Citrate Lyase and does not cross-react with related proteins.

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:3D9-E9-H8

Dilution:WB 1:1000,ICC 1:150,FCM 1:100

Mole Mass:120kDa

Location:Cytoplasm

Concentration:

Sequence Similarity:In the N-terminal section; belongs to the succinate/malate CoA ligase beta subunit family; In the C-terminal section; belongs to the succinate/malate CoA ligase alpha subunit family.

Gene Id:47

SwissProt ID:P53396

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4 150 mM NaCl) with 0.02% sodium azide 50% glycerol

Background:ATP citrate-lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.