Anti-β-Actin-HRP (HRP-Conjugate) Mouse mAb
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Antibody type:Loading control antibody
Label:HRP
Modification:Unmodification
Isotype:IgG2b
Host:Mouse
Application:WB
Purify method:Affinity purified
Species:Human,Mouse,Monkey,Goat,Rat,Hamster,Saccharomyces cerevisiae,Arabidopsis,Pichia pastoris,Rabbit,Zebrafish,Drosophila,Leaf of Rice,Fruit of Cucumber,Chlamydomonas Reinhardtii
Gene Name:beta-actin
Synonyms:BRWS1;PS1TP5BP1
Gene Synonyms:A26C1A;A26C1B;ACTB;ACTB_HUMAN;Actin cytoplasmic 1;Actin,beta;Actin,cytoplasmic 1,N-terminally processed;Actx;Beta actin;beta cytoskeletal actin;Beta-actin;E430023M04Rik; MGC128179;PS1TP5 binding protein 1;PS1TP5BP1.
Gene Full Name:
Gene Infomation:
Antigen:Purified recombinant human beta-actin protein fragments expressed in E.coli.
Antigen Synonyms:
Clonality:Monclonal antibody
Source:Human
Reaction:This antibody detects endogenous levels of beta-actin and does not cross-react with related proteins.
Form:Liquid
Tested Applications:
- Western blot (1:100 to 1:500)
- Immunofluorescence (1:50 to 1:400)
- Immunohistochemistry (1:200 to 1:500)
- Flow cytometry analysis (1:200 to 1:500)
- Enzyme-linked Immunosorbent Assay (1:100-1:5000)
Clone:8F10-G10
Dilution:WB 1:10000
Mole Mass:45kDa
Location:Cytoplasm?cytoskeleton
Concentration:0.2mg/ml
Sequence Similarity:Belongs to the actin family.
Gene Id:60
SwissProt ID:P60709
Unigene:
Nucleotide Accession:
Tissue specificity:
Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Buffer condition:In PBS(pH 7.4) containing with 0.1mg/ml BSA and 50% glycerol.
Background:Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Molar Function:
Western Blot:Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.