Anti-β-Actin Mouse mAb

Antibody type:Loading control antibody

Label:Unconjugated

Modification:Unmodification

Isotype:IgG2b

Host:Mouse

Application:WB

Purify method:Affinity purified

Species:Human,Mouse,Monkey,Goat,Rat,Hamster,Saccharomyces cerevisiae,Arabidopsis,Pichia pastoris,Rabbit,Zebrafish,Drosophila,Leaf of Rice,Fruit of Cucumber,Chlamydomonas Reinhardtii

Gene Name:beta-actin

Synonyms:BRWS1;PS1TP5BP1

Gene Synonyms:A X actin like protein;ACTB;ACTB_HUMAN;Actin;Actin cytoplasmic 1;Actx;b actin;b-actin;Beta cytoskeletal actin;Beta-actin;cytoplasmic 1;Melanoma X actin;N-terminally processed;PS1TP5 binding protein 1;PS1TP5BP1.

Gene Full Name:

Gene Infomation:

Antigen:Purified recombinant human beta actin protein fragments expressed in E.coli.

Antigen Synonyms:

Clonality:Monclonal antibody

Source:Human

Reaction:This antibody detects endogenous levels of beta actin and does not cross-react with related proteins.

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:8F10-G10

Dilution:WB 1:10000,Other 1:5000

Mole Mass:45kDa

Location:Cytoplasm›cytoskeleton

Concentration:

Sequence Similarity:Belongs to the actin family.

Gene Id:60

SwissProt ID:P60709

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Purified mouse monoclonal in PB(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.

Background:Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.