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Anti-β-Actin Mouse mAb

Antibody type:Flowcytometry

Label:Unconjugated

Modification:

Isotype:Unknown

Host:Mouse

Application:IHC-P,FC,WB,IF

Purify method:Affinity purified

Species:Human,Mouse,Rat

Gene Name:beta-actin

Synonyms:Actin, cytoplasmic 1, Beta-actin, Actin, cytoplasmic 1, N-terminally processed, ACTB

Gene Synonyms:

Gene Full Name:

Gene Infomation:This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.

Antigen:This ACTB Monoclonal antibody is generated from mouse immunized with ACTB recombinant protein.

Antigen Synonyms:

Clonality:Monclonal antibody

Source:HUMAN

Reaction:

Form:Liquid

Tested Applications:

  • Western blot (1:100 to 1:500)
  • Immunofluorescence (1:50 to 1:400)
  • Immunohistochemistry (1:200 to 1:500)
  • Flow cytometry analysis (1:200 to 1:500)
  • Enzyme-linked Immunosorbent Assay (1:100-1:5000)
NoteUsers are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:3B12-B7-B7

Dilution:WB::1:1000ICC::1:10~50IHC::1:25FCM::1:25

Mole Mass:42kDa

Location:

Concentration:

Sequence Similarity:

Gene Id:60

SwissProt ID:

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:

Buffer condition:Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Background:

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.