Anti-Akt (pan) Mouse mAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:IgG2b

Host:Mouse

Application:WB

Purify method:Affinity purified

Species:Human,Mouse,Rat,Monkey

Gene Name:Akt

Synonyms:AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA; AKT1.

Gene Synonyms:AKT1 ;AKT1_HUMAN ;AKT2 ;AKT3 ;PKB alpha ;PKB ;PKB beta ;PKBalpha ;PRKBA ;PRKBB ;PRKBG ;Protein kinase B ;Protein kinase B beta ;Protein kinase B gamma ;Proto-oncogene c-Akt ;RAC alpha ;RAC ;RAC gamma ;RAC PK alpha ;RAC PK beta ;RAC PK gamma ;RAC-alpha serine/threonine-protein kinase ;RAC-PK-alpha ;STK2.

Gene Full Name:

Gene Infomation:

Antigen:Purified recombinant human Akt1 protein fragments expressed in E.coli.

Antigen Synonyms:

Clonality:Monclonal antibody

Source:

Reaction:The sequence used to generate this antibody, has a high degree of similarity to regions found in AKT1, AKT2 and AKT3, and thus may cross react with all of these proteins.

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:5A11-C9-G6

Dilution:WB 1:1000

Mole Mass:60kDa

Location:Cytoplasm. Nucleus. Cell membrane. Nucleus after activation by integrin-linked protein kinase 1 (ILK1). Nuclear translocation is enhanced by interaction with TCL1A. Phosphorylation on Tyr-176 by TNK2 results in its localization to the cell membrane where it is targeted for further phosphorylations on Thr-308 and Ser-473 leading to its activation and the activated form translocates to the nucleus.

Concentration:3.1mg/ml

Sequence Similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily.Contains 1 AGC-kinase C-terminal domain.Contains 1 PH domain.Contains 1 protein kinase domain.

Gene Id:207/208/10000

SwissProt ID:P31749P31751Q9Y243

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:PBS(pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.

Background:Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation (By similarity). General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase. The activated form can suppress FoxO gene transcription and promote cell cycle progression. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.