Anti-Akt1 (Phospho-Thr450) Rabbit pAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:

Isotype:Rabbit Ig

Host:Rabbit

Application:DB

Purify method:Affinity purified

Species:Human

Gene Name:akt1

Synonyms:RAC-alpha serine/threonine-protein kinase, Protein kinase B, PKB, Protein kinase B alpha, PKB alpha, Proto-oncogene c-Akt, RAC-PK-alpha, AKT1, PKB, RAC

Gene Synonyms:

Gene Full Name:

Gene Infomation:The serine-threonine protein kinase encoded by the AKT1gene is catalytically inactive in serum-starved primary andimmortalized fibroblasts. AKT1 and the related AKT2 are activatedby platelet-derived growth factor. The activation is rapid andspecific, and it is abrogated by mutations in the pleckstrinhomology domain of AKT1. It was shown that the activation occursthrough phosphatidylinositol 3-kinase. In the developing nervoussystem AKT is a critical mediator of growth factor-induced neuronalsurvival. Survival factors can suppress apoptosis in atranscription-independent manner by activating the serine/threoninekinase AKT1, which then phosphorylates and inactivates componentsof the apoptotic machinery. Multiple alternatively splicedtranscript variants have been found for this gene. [provided byRefSeq].

Antigen:This AKT1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T450 of human AKT1.

Antigen Synonyms:

Clonality:Polyclonal antibody

Source:HUMAN

Reaction:

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:RB29255

Dilution:Other::DB:1:500

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Buffer condition:Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification.

Background:

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Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.