Anti-β-Arrestin 1 Mouse mAb
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Antibody type:Primary antibody
Label:Unconjugated
Modification:Unmodification
Isotype:IgG2b
Host:Mouse
Application:WB
Purify method:Affinity purified
Species:Mouse,Rat,Human
Gene Name:arrb1
Synonyms:ARB1;ARR1.
Gene Synonyms:ARB 1;ARB 1;ARB1;ARB1;ARR 1;ARR 1;ARR1;ARR1;ARRB 1;ARRB1;ARRB1;ARRB1_HUMAN;Arrestin 2; Arrestin beta 1;Arrestin beta-1;Beta-arrestin-1.
Gene Full Name:
Gene Infomation:
Antigen:Purified recombinant human beta Arrestin 1 protein fragments expressed in E.coli.
Antigen Synonyms:
Clonality:Monclonal antibody
Source:Human
Reaction:This antibody detects endogenous levels of beta Arrestin 1 and does not cross-react with related proteins.
Form:Liquid
Tested Applications:
- Western blot (1:100 to 1:500)
- Immunofluorescence (1:50 to 1:400)
- Immunohistochemistry (1:200 to 1:500)
- Flow cytometry analysis (1:200 to 1:500)
- Enzyme-linked Immunosorbent Assay (1:100-1:5000)
Clone:1H4-F5-C11
Dilution:WB 1:500
Mole Mass:50kDa
Location:Cytoplasm.Nucleus.Cell membrane.Membrane ? clathrin-coated pit.Cell projection ? pseudopodium.Cytoplasmic vesicle.
Concentration:
Sequence Similarity:Belongs to the arrestin family.
Gene Id:408
SwissProt ID:P49407
Unigene:
Nucleotide Accession:
Tissue specificity:
Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Buffer condition:Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine(pH 7.4 150 mM NaCl)with 0.02% sodium azide 0.1mg/mlBSA and 50% glycerol.
Background:Arrestin proteins function as negative regulators of G protein-coupled receptor (GPCR) signaling. Cognate ligand binding stimulates GPCR phosphorylation, which is followed by binding of arrestin to the phosphorylated GPCR and the eventual internalization of the receptor and desensitization of GPCR signaling. Four distinct mammalian arrestin proteins are known. Arrestin 1 (also known as S-arrestin) and arrestin 4 (X-arrestin) are localized to retinal rods and cones, respectively.
Molar Function:
Western Blot:Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.