Anti-B-RAF(S445) Rabbit pAb

100ug (NP27206) Please inquiry

Antibody type:Flowcytometry

Label:Unconjugated

Modification:

Isotype:Rabbit Ig

Host:Rabbit

Application:WB,IHC-P,IF,FC

Purify method:Affinity purified

Species:Human

Gene Name:b-raf

Synonyms:Serine/threonine-protein kinase B-raf, Proto-oncogene B-Raf, p94, v-Raf murine sarcoma viral oncogene homolog B1, BRAF, BRAF1, RAFB1

Gene Synonyms:

Gene Full Name:

Gene Infomation:BRAF, a member of the RAF subfamily of Ser/Thr protein kinases, is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It may play a role in the postsynaptic responses of hippocampal neurons. This cytoplasmic protein is expressed in brain and testis.Defects in BRAF are involved in a wide range of cancers including lung cancer and non-Hodgkin lymphoma (NHL).This protein contains 1 zinc-dependent phorbol-ester and DAG binding domain.

Antigen:This B-RAF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 424-453 amino acids from human B-RAF.

Antigen Synonyms:

Clonality:Polyclonal antibody

Source:HUMAN

Reaction:

Form:Liquid

Tested Applications:

Western blot (1:100 to 1:500)
Immunofluorescence (1:50 to 1:400)
Immunohistochemistry (1:200 to 1:500)
Flow cytometry analysis (1:200 to 1:500)
Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:RB10940,RB17180

Dilution:WB::1:1000ICC::1:10~50IHC::1:10~50FCM::1:10~50

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Storage:

Buffer condition:Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Background:

Molar Function:

Western Blot:

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.