Anti-B Raf Mouse mAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:IgG2a

Host:Mouse

Application:WB

Purify method:Affinity purified

Species:Human,Mouse

Gene Name:braf

Synonyms:BRAF NS7; BRAF1; RAFB1; B-RAF1; B Raf

Gene Synonyms:FLJ95109;94 kDa B raf protein;B raf 1;B Raf proto oncogene serine threonine protein kinase;B-Raf proto-oncogene serine/threonine-protein kinase (p94);BRAF 1;BRAF;BRAF_HUMAN;BRAF1;cRmil;MGC126806;MGC138284;Murine sarcoma viral (v-raf) oncogene homolog B1;M

Gene Full Name:

Gene Infomation:

Antigen:Purified recombinant human B Raf protein fragments expressed in E.coli.

Antigen Synonyms:

Clonality:Monclonal antibody

Source:Human

Reaction:This antibody detects endogenous levels of B Raf and does not cross-react with related proteins.

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:4E1-E1-F9

Dilution:WB 1:1000

Mole Mass:87kDa

Location:Nucleus. Cytoplasm. Cell membrane

Concentration:

Sequence Similarity:Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily. Contains 1 phorbol-ester/DAG-type zinc finger. Contains 1 protein kinase domain. Contains 1 RBD (Ras-binding) domain.

Gene Id:673

SwissProt ID:P15056

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.

Background:Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.