Anti-BAD Rabbit pAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:

Isotype:IgG

Host:Rabbit

Application:WB,ICC,IHC

Purify method:Affinity purified

Species:Human,Mouse,Rat

Gene Name:bad

Synonyms:BAD;BAD_HUMAN;BBC 2;BBC2;BBC6;Bcl 2 Antagonist of Cell Death;Bcl 2 Binding Component 6;BCL X / BCL 2 Binding Protein;BCL X Binding Protein;Bcl XL/Bcl 2 Associated Death Promoter;Bcl-2-binding component 6;Bcl-2-like protein 8;Bcl-XL/Bcl-2-associated death promoter;Bcl2 antagonist of cell death;BCL2 antagonist of cell death protein;BCL2 associated agonist of cell death;Bcl2 Associated Death Promoter;BCL2 binding component 6;BCL2 binding protein;Bcl2 Like 8 Protein;Bcl2-L-8;BCL2L8;Proapoptotic BH3 Only Protein.

Gene Synonyms:BBC2; BCL2L8

Gene Full Name:

Gene Infomation:The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform. [provided by RefSeq, Jul 2008]

Antigen:The antiserum was produced against synthesized non-phosphopeptide derived from human BAD around the phosphorylation site of serine 112.

Antigen Synonyms:

Clonality:Polyclonal antibody

Source:Human

Reaction:

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:

Mole Mass:18

Location:Mitochondrion outer membrane. Cytoplasm. Note: Upon phosphorylation, locates to the cytoplasm.

Concentration:

Sequence Similarity:Belongs to the Bcl-2 family.

Gene Id:572

SwissProt ID:

Unigene:

Nucleotide Accession:

Tissue specificity:This antibody detects endogenous levels of Bad and does not cross-react with related proteins.

Storage:

Buffer condition:10mM PBS with 0.02% sodium azide, 50% glycerol.

Background:

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.