Anti-BORA Rabbit pAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:Rabbit IgG

Host:Rabbit

Application:ELISA,WB,IHC

Purify method:Affinity purified

Species:Human

Gene Name:BORA

Synonyms:C13orf34

Gene Synonyms:BORA

Gene Full Name:

Gene Infomation:

Antigen:Synthetic peptide of human BORA

Antigen Synonyms:C13orf34

Clonality:Polyclonal antibody

Source:Human

Reaction:

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:WB::500-2000

Mole Mass:61 kDa

Location:

Concentration:

Sequence Similarity:

Gene Id:

SwissProt ID:

Unigene:

Nucleotide Accession:NP_079084

Tissue specificity:

Storage:

Buffer condition:-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Background:

Molar Function:Aurora related kinase-1 (ARK-1) is a centrosome-associated serine/threonine kinase that regulates centrosome separation, bipolar spindle assembly and chromosome segregation during mitosis. Bora (protein aurora borealis) is a 559 amino acid protein that activates ARK-1. Bora is localized to the nucleus until mitosis is initiated, when it then translocates to the cytoplasm. This translocation is dependent on activated Cdc2, which releases Bora to bind and activate ARK-1 in the cytoplasm. Plk (polo-like kinase) interacts with Bora to control the accessibility of its activation loop for phosphorylation and activation on its N-terminus by ARK-1. It is through this mechanism that Bora and ARK-1 control cellular mitotic entry. Downregulation of the gene encoding Bora results in multipolar spindles in mitosis, a phenomenon that is also observed when ARK-1 function is blocked.

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.