Anti-BRCA1 Rabbit pAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:

Isotype:IgG

Host:Rabbit

Application:WB,ICC,IHC

Purify method:Affinity purified

Species:Human

Gene Name:brca1

Synonyms:BRAC 1;BRCA 1;BRCA1;BRCA1/BRCA2 containing complex subunit 1;BRCA1/BRCA2-containing complex, subunit 1;BRCA1_HUMAN;BRCAI;BRCC 1;BRCC1;Breast and ovarian cancer susceptibility protein 1;Breast Cancer 1;Breast Cancer 1 Early Onset;Breast cancer type 1 susceptibility protein;Breast Ovarian Cancer Susceptibility;Breast Ovarian Cancer Susceptibility;BROVCA1;IRIS;Papillary Serous Carcinoma Of The Peritoneum;PNCA4;PPP1R53;Protein phosphatase 1 regulatory subunit 53;PSCP;RING finger protein 53;RING finger protein 53;RNF53.

Gene Synonyms:IRIS; PSCP; BRCAI; BRCC1; PNCA4; RNF53; BROVCA1; PPP1R53

Gene Full Name:

Gene Infomation:This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified. [provided by RefSeq, May 2009]

Antigen:The antiserum was produced against synthesized non-phosphopeptide derived from human BRCA1 around the phosphorylation site of serine 1524.

Antigen Synonyms:

Clonality:Polyclonal antibody

Source:Human

Reaction:

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:

Mole Mass:207

Location:Nucleus. Chromosome. Note: Localizes at sites of DNA damage at double-strand breaks (DSBs); recruitment to DNA damage sites is mediated by the BRCA1-A complex.Isoform 3: Cytoplasm .Isoform 5: Cytoplasm.

Concentration:

Sequence Similarity:Contains 2 BRCT domains.Contains 1 RING-type zinc finger.

Gene Id:672

SwissProt ID:

Unigene:

Nucleotide Accession:

Tissue specificity:This antibody detects endogenous levels of BRCA1 and does not cross-react with related proteins.

Storage:

Buffer condition:10mM PBS with 0.02% sodium azide, 50% glycerol.

Background:

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.