Anti-Bcl-2 Mouse mAb (3H5)

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:IgG1

Host:Mouse

Application:WB,IHC,IF

Purify method:Affinity purified

Species:Human,Rat,Mouse

Gene Name:BCL2

Synonyms:Apoptosis regulator Bcl 2, Bcl 2, B cell leukemia/lymphoma 2

Gene Synonyms:

Gene Full Name:

Gene Infomation:

Antigen:Synthetic peptide conjugated to KLH.

Antigen Synonyms:

Clonality:Monclonal antibody

Source:Mouse

Reaction:The Bcl-2 mouse monoclonal antibody candetectendogenous Bcl-2 proteins.

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:3H5-2H8-1H5

Dilution:WB 1:1,000-2,000 IHC 1:200 IF 1:100-200 (Optimal dilutions should be determined by the end user)

Mole Mass:26kDa

Location:Endoplasmic reticulum, Membrane, Mitochondrion, Mitochondrion outer membrane, Nucleus

Concentration:

Sequence Similarity:

Gene Id:596

SwissProt ID:P10415

Unigene:

Nucleotide Accession:596

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.

Background:Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.