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Anti-Bcl2 Rabbit pAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:

Isotype:IgG

Host:Rabbit

Application:WB

Purify method:Affinity purified

Species:Human,Monkey

Gene Name:bcl2

Synonyms:Apoptosis regulator Bcl 2;Apoptosis regulator Bcl-2;Apoptosis regulator Bcl2;AW986256;B cell CLL/lymphoma 2;B cell leukemia/lymphoma 2;B cell lymphoma 2;Bcl 2;Bcl-2;Bcl2;BCL2 protein;BCL2_HUMAN;C430015F12Rik;D630044D05Rik;D830018M01Rik;Leukemia/lymphoma, B-cell, 2;Oncogene B-cell leukemia 2;PPP1R50;Protein phosphatase 1, regulatory subunit 50.

Gene Synonyms:Bcl-2; PPP1R50

Gene Full Name:

Gene Infomation:This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq, Jul 2008]

Antigen:Peptide sequence around aa. 54~58 (G-H-T-P-H) derived from human Bcl2.

Antigen Synonyms:

Clonality:Polyclonal antibody

Source:Human

Reaction:

Form:Liquid

Tested Applications:

  • Western blot (1:100 to 1:500)
  • Immunofluorescence (1:50 to 1:400)
  • Immunohistochemistry (1:200 to 1:500)
  • Flow cytometry analysis (1:200 to 1:500)
  • Enzyme-linked Immunosorbent Assay (1:100-1:5000)
NoteUsers are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:WB::1:300

Mole Mass:26

Location:Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane;

Concentration:1mg/ml

Sequence Similarity:Belongs to the Bcl-2 family.

Gene Id:596

SwissProt ID:

Unigene:

Nucleotide Accession:

Tissue specificity:The antibody detects endogenous level of total Bcl2 protein.

Storage:

Buffer condition:Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Background:

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.