Anti-BiP/GRP78(C-term) Mouse mAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:IgG1

Host:Mouse

Application:WB,ICC,IHC

Purify method:Affinity purified

Species:Human,Rat

Gene Name:hspa5

Synonyms:BIP; MIF2; GRP78

Gene Synonyms:78 kDa glucose regulated protein;78 kDa glucose-regulated protein;AL022860;AU019543;BIP; D2Wsu141e;D2Wsu17e;Endoplasmic reticulum lumenal Ca(2 )-binding protein grp78; Endoplasmic reticulum lumenal Ca2binding protein grp78;FLJ26106;Glucose Regulated Pro

Gene Full Name:

Gene Infomation:

Antigen:Purified recombinant human BiP/GRP78 (C-terminus) protein fragments expressed in E.coli.

Antigen Synonyms:

Clonality:Monclonal antibody

Source:Human

Reaction:This antibody detects endogenous levels of BiP/GRP78 (C-terminus) and does not cross-react with related proteins.

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:9E4-2A7-H6

Dilution:WB 1:1000,ICC 1:50

Mole Mass:78kDa

Location:Endoplasmic reticulum lumen. Melanosome

Concentration:

Sequence Similarity:Belongs to the heat shock protein 70 family

Gene Id:3309

SwissProt ID:P11021

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.

Background:When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) (PubMed 8020977) pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER).

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.