Anti-BiP/GRP78 Mouse mAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:IgG2b

Host:Mouse

Application:WB,IF,IP

Purify method:Affinity purified

Species:Human,Mouse,Rat

Gene Name:hspa5

Synonyms:BIP; MIF2; GRP78; HEL-S-89n.

Gene Synonyms:78 kDa glucose regulated protein;78 kDa glucose-regulated protein;AL022860;AU019543;BIP;D2Wsu141e;D2Wsu17e;Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78;Endoplasmic reticulum lumenal Ca2+ binding protein grp78;FLJ26106;Glucose Regulated Prote

Gene Full Name:

Gene Infomation:

Antigen:Purified recombinant human BiP/GRP78 protein expressed in E.coli.

Antigen Synonyms:

Clonality:Monclonal antibody

Source:Human

Reaction:This antibody detects endogenous levels of BiP/GRP78 and does not cross-react with related proteins.

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:4F11-D5-C10

Dilution:WB 1:1000

Mole Mass:78kDa

Location:Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Concentration:

Sequence Similarity:Belongs to the heat shock protein 70 family.

Gene Id:3309

SwissProt ID:P11021

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:PBS(pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.

Background:Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.