Anti-CD31 Mouse mAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:IgG1

Host:Mouse

Application:WB,IP

Purify method:Affinity purified

Species:Human

Gene Name:pecam1

Synonyms:Cd31;Pecam;C85791;PECAM-1.

Gene Synonyms:Adhesion molecule;CD31;CD31 antigen;CD31 EndoCAM;Endocam;FLJ34100;FLJ58394;GPIIA; GPIIA';PECA1;PECA1_HUMAN;Pecam 1;PECAM 1 CD31 EndoCAM;PECAM;PECAM-1;Pecam1;Platelet endothelial cell adhesion molecule;Platelet/endothelial cell adhesion molecule 1; Platele

Gene Full Name:

Gene Infomation:

Antigen:Purified recombinant human CD31 protein fragments expressed in E.coli.

Antigen Synonyms:

Clonality:Monclonal antibody

Source:Human

Reaction:This antibody detects endogenous levels of CD31 and does not cross-react with related proteins.

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:2D3-B4-C8

Dilution:WB 1:1000

Mole Mass:130kDa

Location:Isoform Long: Membrane;Single-pass type I membrane protein.Cell junction.Isoform Delta15: Cell junction.

Concentration:

Sequence Similarity:Contains 6 Ig-like C2-type (immunoglobulin-like) domains.

Gene Id:18613

SwissProt ID:P16284

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide 0.1mg/mlBSA and 50% glycerol.

Background:Induces susceptibility to atherosclerosis By similarity.Cell adhesion molecule which is required for leukocyte transendothelial migration(TEM)under most inflammatory conditions.Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment(LBRC)and is also essential for the LBRC membrane to be targeted around migrating leukocytes.Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals,and changes function on apoptosis,promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte.During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore.The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment).Isoform Delta15 is unable to protect against apoptosis.Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells(HUVEC).

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.