Anti-CDK4 Mouse mAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:IgG1

Host:Mouse

Application:WB

Purify method:Affinity purified

Species:Human

Gene Name:CDK4

Synonyms:CDK4;CMM3;PSK-J3.

Gene Synonyms:Cdk4;cdk4;CDK4protein;CDK4_HUMAN;Celldivisionkinase4;Celldivisionproteinkinase4;CMM3;CMM3;Crk3;Cyclindependentkinase4;Cyclin-dependentkinase4;Melanomacutaneousmalignant3;MGC14458;p34cdk4;PSKJ3;PSK-J3.

Gene Full Name:

Gene Infomation:

Antigen:Purified recombinant human CDK4 protein expressed in E.coli.

Antigen Synonyms:

Clonality:Monclonal antibody

Source:

Reaction:CDK4 Mouse mAb recognizes endogenous levels of total CDK4 protein.

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:3D12-A7-F11

Dilution:WB 1:500

Mole Mass:34kDa

Location:Cytoplasm_x000D_Nucleus_x000D_Membrane

Concentration:3.86mg/ml

Sequence Similarity:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily._x000D_Contains 1 protein kinase domain.

Gene Id:1019

SwissProt ID:P11802

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.

Background:Cyclin-dependent kinase activity is regulated by T-loop phosphorylation (Thr172 in the case of CDK4), by the abundance of their cyclin partners, and by association with CDK inhibitors of the Cip/Kip or INK family of proteins. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.