Anti-CDK4 Rabbit pAb
- 100ug (NP21198) Please inquiry
Antibody type:Primary antibody
Label:Unconjugated
Modification:Unmodification
Isotype:Rabbit IgG
Host:Rabbit
Application:WB,IHC,ICC/IF,FC
Purify method:Affinity Chromatography
Species:Human
Gene Name:
Synonyms:Cyclin-dependent kinase 4; Cell division protein kinase 4; PSK-J3; PSKJ3; CDK4; CMM 3;
Gene Synonyms:
Gene Full Name:
Gene Infomation:Associates with the spliceosome late in the splicing pathway and may function in the second step of pre-mRNA splicing.
Antigen:A synthesized peptide derived from human CDK4
Antigen Synonyms:
Clonality:Polyclonal antibody
Source:
Reaction:CDK4 Antibody detects endogenous levels of CDK4
Form:Liquid
Tested Applications:
- Western blot (1:100 to 1:500)
- Immunofluorescence (1:50 to 1:400)
- Immunohistochemistry (1:200 to 1:500)
- Flow cytometry analysis (1:200 to 1:500)
- Enzyme-linked Immunosorbent Assay (1:100-1:5000)
Clone:
Dilution:WB 1:1000~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 FC 1:50
Mole Mass:34kDa
Location:Nucleus;
Concentration:
Sequence Similarity:
Gene Id:
SwissProt ID:P11802
Unigene:
Nucleotide Accession:
Tissue specificity:CDK4 Antibody detects endogenous levels of CDK4
Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Buffer condition:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Background:Cyclin-dependent kinase activity is regulated by T-loop phosphorylation (Thr172 in the case of CDK4), by the abundance of their cyclin partners, and by association with CDK inhibitors of the Cip/Kip or INK family of proteins. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals.
Molar Function:
Western Blot:Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.