Anti-CHD3(C-term) Mouse mAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:IgG1

Host:Mouse

Application:WB,IHC,FC

Purify method:Affinity purified

Species:Human

Gene Name:chd3

Synonyms:ZFH; Mi-2a; Mi2-ALPHA

Gene Synonyms:226;2600010P09Rik;AF020312;ATP dependent helicase CHD3;ATP-dependent helicase Chd3;CHD-3;Chd3;CHD3_HUMAN;Chd7;Chromodomain-helicase-DNA-binding protein 3;hZFH;MGC40857;Mi 2 autoantigen 240 kDa protein;Mi 2a;Mi-2 autoantigen 240 kDa protein;Mi2 ALPHA;Mi2-a

Gene Full Name:

Gene Infomation:

Antigen:Purified recombinant human CHD3 (C-terminus) protein fragments expressed in E.coli.

Antigen Synonyms:

Clonality:Monclonal antibody

Source:Human

Reaction:This antibody detects endogenous levels of CHD3 (C-terminus) and does not cross-react with related proteins.

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:4B1-F1-G4

Dilution:WB 1:1000,IHC 1:500~1:1000,FCM 1:100

Mole Mass:260kDa

Location:Nucleus. Cytoplasm

Concentration:

Sequence Similarity:Belongs to the SNF2/RAD54 helicase family._x000D_Contains 2 chromo domains._x000D_Contains 1 helicase ATP-binding domain._x000D_Contains 1 helicase C-terminal domain._x000D_Contains 2 PHD-type zinc fingers

Gene Id:1107

SwissProt ID:Q12873

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4 150 mM NaCl) with 0.02% sodium azide 50% glycerol

Background:Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Required for anchoring centrosomal pericentrin in both interphase and mitosis, for spindle organization and centrosome integrity.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.