Anti-CHK2(Phospho-Thr68) Rabbit pAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:

Isotype:IgG

Host:Rabbit

Application:WB,IHC

Purify method:Affinity purified

Species:Human,Mouse,Rat

Gene Name:chek2

Synonyms:bA444G7;CDS 1;CDS1;Cds1 homolog;Checkpoint kinase 2;Checkpoint like protein CHK2;Chek 2;Chek2;Chk 2;CHK2 checkpoint homolog (S. pombe);CHK2 checkpoint homolog;CHK2_HUMAN;hCds1;HuCds 1;HuCds1;LFS 2;LFS2;PP1425;RAD 53;RAD53;Rad53 homolog;Serine/threonine protein kinase Chk2;Serine/threonine-protein kinase Chk2.

Gene Synonyms:CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425

Gene Full Name:

Gene Infomation:In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

Antigen:The antiserum was produced against synthesized phosphopeptide derived from human CHK2 around the phosphorylation site of threonine 68 (V-S-TP-Q-E).

Antigen Synonyms:

Clonality:Polyclonal antibody

Source:Human

Reaction:

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:

Mole Mass:61

Location:Isoform 2: Nucleus . Note: Isoform 10 is present throughout the cell. Isoform 4: Nucleus.Isoform 7: Nucleus.Isoform 9: Nucleus.Isoform 12: Nucleus.Nucleus › PML body. Nucleus › nucleoplasm. Note: Recruited into PML bodies together with TP53.

Concentration:

Sequence Similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHK2 subfamily.Contains 1 FHA domain.Contains 1 protein kinase domain.

Gene Id:11200

SwissProt ID:

Unigene:

Nucleotide Accession:

Tissue specificity:This antibody detects endogenous levels of Chk2 and does not cross-react with related proteins.

Storage:

Buffer condition:10mM PBS with 0.02% sodium azide, 50% glycerol.

Background:

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.