Anti-CHOP Mouse(2B1) Mouse mAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:

Isotype:IgG

Host:Mouse

Application:WB,IF,IHC

Purify method:Affinity Chromatography

Species:Human,Mouse,Rat

Gene Name:DDIT3

Synonyms:DDIT3

Gene Synonyms:CHOP; CEBPZ; CHOP10; CHOP-10; GADD153

Gene Full Name:

Gene Infomation:This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified. [provided by RefSeq, Aug 2010]

Antigen:Synthetic Peptide of CHOP at AA range of 10-90

Antigen Synonyms:

Clonality:Monclonal antibody

Source:

Reaction:

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:WB::1:1000-2000ICC::1:200IHC::1:100-200

Mole Mass:27

Location:Nucleus;

Concentration:

Sequence Similarity:Contains 1 bZIP (basic-leucine zipper) domain.

Gene Id:1649

SwissProt ID:

Unigene:

Nucleotide Accession:

Tissue specificity:CHOP protein detects endogenous levels of DDIT3

Storage:

Buffer condition:Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Background:

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.