Anti-CKII alpha prime polypeptide Mouse mAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:Mouse IgG1

Host:Mouse

Application:WB,FCM

Purify method:Ascitic fluid

Species:Human,Mouse

Gene Name:CSNK2A2

Synonyms:CK2A2; CSNK2A1; FLJ43934

Gene Synonyms:

Gene Full Name:

Gene Infomation:

Antigen:Purified recombinant fragment of human CSNK2A2 (AA: 194-350) expressed in E. Coli.

Antigen Synonyms:

Clonality:Monclonal antibody

Source:

Reaction:

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:5F9-H11-F7

Dilution:WB: 1/500 - 1/2000; IHC: N/A; ICC: N/A; FCM: 1/200 - 1/400; Elisa: 1/10000

Mole Mass:41.2kDa

Location:

Concentration:

Sequence Similarity:

Gene Id:1459

SwissProt ID:P19784

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Ascitic fluid containing 0.03% sodium azide.

Background:Casein kinase II (CK2) is a constitutively active, ubiquitously expressed serine/threonine protein kinasethat is thought to have a regulatory function in cell proliferation, cell differentiation and apoptosis. CK2functions as a tetrameric complex consisting of two regulatory beta-subunits and two catalytic units (alphaand alpha') in a homomeric or heteromeric conformation. Whilst the alpha- and alpha'-subunits arecatalytically identical, proteins that regulate CK2, such as cdc2 and Hsp90, preferentially bind to thealpha and not the alpha'-subunit. CK2 can phosphorylate a number of key intracellular signaling proteinsimplicated in tumor suppression (p53 and PTEN) and tumorigenesis (myc, jun, NF-kappaB). CK2 is also thoughtto influence Wnt signaling via beta-catenin phosphorylation and the PI 3-K signaling pathway via thephosphorylation of Akt.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.