Anti-CKMT2 Mouse mAb
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Antibody type:Primary antibody
Label:Unconjugated
Modification:Unmodification
Isotype:IgG2b
Host:Mouse
Application:WB
Purify method:Affinity purified
Species:Rat
Gene Name:ckmt2
Synonyms:SMTCK
Gene Synonyms:CKMT 2;Basic-type mitochondrial creatine kinase;CKMT 2;CKMT2;CPK;Creatine kinase mitochondrial 2;Creatine kinase mitochondrial 2 (sarcomeric);Creatine kinase S-type; creatine kinase S-type, mitochondrial;KCRS_HUMAN;Mib CK;Mib-CK;mitochondrial; OTTHUMP0000
Gene Full Name:
Gene Infomation:
Antigen:Purified recombinant human CKMT2 protein fragments expressed in E.coli.
Antigen Synonyms:
Clonality:Monclonal antibody
Source:Human
Reaction:This antibody detects endogenous levels of CKMT2 and does not cross-react with related proteins.
Form:Liquid
Tested Applications:
- Western blot (1:100 to 1:500)
- Immunofluorescence (1:50 to 1:400)
- Immunohistochemistry (1:200 to 1:500)
- Flow cytometry analysis (1:200 to 1:500)
- Enzyme-linked Immunosorbent Assay (1:100-1:5000)
Clone:3F4-G5-H5
Dilution:WB 1:1000
Mole Mass:47kDa
Location:Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane side.
Concentration:
Sequence Similarity:Belongs to the ATP:guanido phosphotransferase family._x000D__x000D_Contains 1 phosphagen kinase C-terminal domain._x000D__x000D_Contains 1 phosphagen kinase N-terminal domain.
Gene Id:1160
SwissProt ID:P17540
Unigene:
Nucleotide Accession:
Tissue specificity:
Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Buffer condition:Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4 150 mM NaCl) with 0.02% sodium azide 50% glycerol
Background:Mitochondrial creatine kinase (MtCK) is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Sarcomeric mitochondrial creatine kinase has 80% homology with the coding exons of ubiquitous mitochondrial creatine kinase. This gene contains sequences homologous to several motifs that are shared among some nuclear genes encoding mitochondrial proteins and thus may be essential for the coordinated activation of these genes during mitochondrial biogenesis. Three transcript variants encoding the same protein have been found for this gene.
Molar Function:
Western Blot:Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.