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Anti-CRISPR-Cas9 SP Rabbit pAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:Rabbit IgG

Host:Rabbit

Application:WB,IHC,ICC/IF,FC

Purify method:Affinity Chromatography

Species:Streptococcus Pyogenes

Gene Name:

Synonyms:Cas9; CRISPR-associated endonuclease Cas9/Csn1; CRISPR-Cas9/Csn1; csn1; SpyCas9;

Gene Synonyms:

Gene Full Name:

Gene Infomation:The CRISPR associated protein 9 (Cas9) is an RNA-guided DNA nuclease and part of the Streptococcus pyogenes CRISPR antiviral immunity system that provides adaptive immunity against extra chromosomal genetic material. CRISPR/Cas9 genome editing tools have been used in many organisms, including mouse and human cells.

Antigen:A synthesized peptide derived from human CRISPR-Cas9 SP

Antigen Synonyms:

Clonality:Polyclonal antibody

Source:

Reaction:CRISPR-Cas9 SP Antibody detects endogenous levels of total CRISPR-Cas9 SP

Form:Liquid

Tested Applications:

  • Western blot (1:100 to 1:500)
  • Immunofluorescence (1:50 to 1:400)
  • Immunohistochemistry (1:200 to 1:500)
  • Flow cytometry analysis (1:200 to 1:500)
  • Enzyme-linked Immunosorbent Assay (1:100-1:5000)
NoteUsers are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:WB 1:1000~1:5000 IHC 1:50~1:200 ICC/IF 1:50~1:200 FC 1:60

Mole Mass:158kDa

Location:

Concentration:

Sequence Similarity:Belongs to the CRISPR-associated protein Cas9 family. Subtype II-A subfamily.

Gene Id:

SwissProt ID:Q99ZW2

Unigene:

Nucleotide Accession:

Tissue specificity:CRISPR-Cas9 SP Antibodydetects endogenous levels of total CRISPR-Cas9 SP

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Background:The CRISPR associated protein 9 (Cas9) is an RNA-guided DNA nuclease and part of the Streptococcus pyogenes CRISPR antiviral immunity system that provides adaptive immunity against extra chromosomal genetic material. CRISPR/Cas9 genome editing tools have been used in many organisms, including mouse and human cells.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.