Anti-CSK Mouse mAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:IgG2b

Host:Mouse

Application:WB,ICC

Purify method:Affinity purified

Species:Human,Rat

Gene Name:csk

Synonyms:C SRC;C SRC kinase;C src Tyrosine Kinase;C-SRC kinase;c-src tyrosine kinase;Csk A;CSK; CSK_HUMAN;CYTOPLASMIC TYROSINE KINASE;EC 2.7.10.2;MGC112926;MGC117393;MGC154049;P60 Src; Protein tyrosine kinase CYL;Protein-tyrosine kinase CYL;Proto oncogene tyrosine protein kinase;Tyrosine protein kinase CSK;Tyrosine protein kinase CSK;Tyrosine-protein kinase CSK;zgc:154049.

Gene Synonyms:C SRC;C SRC kinase;C src Tyrosine Kinase;C-SRC kinase;c-src tyrosine kinase;Csk A;CSK; CSK_HUMAN;CYTOPLASMIC TYROSINE KINASE;EC 2.7.10.2;MGC112926;MGC117393;MGC154049;P60 Src; Protein tyrosine kinase CYL;Protein-tyrosine kinase CYL;Proto oncogene tyrosine

Gene Full Name:

Gene Infomation:

Antigen:Purified recombinant human CSK protein fragments expressed in E.coli.

Antigen Synonyms:

Clonality:Monclonal antibody

Source:Human

Reaction:This antibody detects endogenous levels of CSK and does not cross-react with related proteins.

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:2B4-C6-A10

Dilution:WB 1:1000,ICC 1:50

Mole Mass:50kDa

Location:Cytoplasm. Cell membrane.

Concentration:

Sequence Similarity:Belongs to the protein kinase superfamily.Tyr protein kinase family.CSK subfamily.Contains 1 protein kinase domain.Contains 1 SH2 domain.Contains 1 SH3 domain.

Gene Id:1445

SwissProt ID:P41240

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine(pH 7.4 150 mM NaCl)with 0.02% sodium azide 0.1mg/mlBSA and 50% glycerol.

Background:CSK(Tyrosine-protein kinase CSK) is also named as C-Src kinase, protein-tyrosine kinase CYL and belongs to the protein kinase superfamily. Csk is a non-receptor tyrosine kinase with a molecular mass of 50 kDa. It contains the SH3 and SH2 domains in its N-terminus and a kinase domain in its C-terminus. It negatively regulates the kinase activity of SFKs and plays an important role in physiological functions via signaling pathways for cell proliferation, differentiation, adhesion, and migration(PMID:23548896). Csk may play a role in tumor suppression by inhibiting the oncogenic activity of SFKs. Indeed, overexpression of Csk can inhibit tumor growth of human colon cancer cells(PMID:23139636). This protein can be autophosphorylated(PMID:11181701). This antibody is specific to CSK.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.