Anti-CYP51A1 Rabbit pAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:Rabbit IgG

Host:Rabbit

Application:IF

Purify method:Affinity purified

Species:Human

Gene Name:CYP51A1

Synonyms:LDM; CP51; CYP51; CYPL1; P450L1; P450-14DM

Gene Synonyms:

Gene Full Name:cytochrome P450 family 51 subfamily A member 1

Gene Infomation:This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein participates in the synthesis of cholesterol by catalyzing the removal of the 14alpha-methyl group from lanosterol. Homologous genes are found in all three eukaryotic phyla, fungi, plants, and animals, suggesting that this is one of the oldest cytochrome P450 genes. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Antigen:Recombinant fusion protein containing a sequence corresponding to amino acids 210-509 of human CYP51A1 (NP_000777.1).

Antigen Synonyms:Lanosterol 14-alpha demethylase

Clonality:Polyclonal antibody

Source:Human

Reaction:

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:IF1:50 -1:200(Optimal dilutions should be determined by the end user)

Mole Mass:46kDa/56kDa

Location:Endoplasmic reticulum, Membrane, Microsome

Concentration:

Sequence Similarity:

Gene Id:

SwissProt ID:Q16850

Unigene:1595

Nucleotide Accession:

Tissue specificity:Ubiquitously expressed with highest levels in testis, ovary, adrenal, prostate, liver, kidney and lung.

Storage:Store at -20°C. Avoid freeze / thaw cycles.

Buffer condition:PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Background:

Molar Function:Catalyzes C14-demethylation of lanosterol; it transforms lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol.

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.