Anti-Caspase-3 Mouse mAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:Mouse IgG1

Host:Mouse

Application:WB,IHC,ICC,FCM

Purify method:Affinity purified

Species:Human

Gene Name:CASP3

Synonyms:CPP32; SCA-1; CPP32B

Gene Synonyms:

Gene Full Name:

Gene Infomation:

Antigen:Purified recombinant fragment of human CASP3 (AA: 29-175) expressed in E. Coli.

Antigen Synonyms:

Clonality:Monclonal antibody

Source:

Reaction:

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:6C12-F1-G3

Dilution:WB: 1/500 - 1/2000; IHC: ; ICC: ; FCM: 1/200 - 1/400; Elisa: 1/10000

Mole Mass:31.6kDa

Location:

Concentration:

Sequence Similarity:

Gene Id:836

SwissProt ID:P42574

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Purified antibody in PBS with 0.05% sodium azide

Background:This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.