Anti-Caspase-9 Mouse mAb
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Antibody type:Primary antibody
Label:Unconjugated
Modification:Unmodification
Isotype:IgG1
Host:Mouse
Application:WB
Purify method:Affinity purified
Species:Human,Transfected
Gene Name:casp9
Synonyms:CASP9; MCH6; APAF3; APAF-3; PPP1R56; ICE-LAP6
Gene Synonyms:APAF 3;APAF-3;APAF3;Apoptosis related cysteine peptidase;Apoptotic protease activating factor 3;Apoptotic protease Mch 6;Apoptotic protease Mch-6;Apoptotic protease-activating factor 3;CASP 9;CASP-9;CASP9;CASP9_HUMAN;Caspase 9 apoptosis related cysteine p
Gene Full Name:
Gene Infomation:
Antigen:Purified recombinant human Caspase-9 protein fragments expressed in E.coli.
Antigen Synonyms:
Clonality:Monclonal antibody
Source:Human
Reaction:This antibody detects endogenous levels of Caspase-9 and does not cross-react with related proteins.
Form:Liquid
Tested Applications:
- Western blot (1:100 to 1:500)
- Immunofluorescence (1:50 to 1:400)
- Immunohistochemistry (1:200 to 1:500)
- Flow cytometry analysis (1:200 to 1:500)
- Enzyme-linked Immunosorbent Assay (1:100-1:5000)
Clone:1D1-F2-E9
Dilution:WB 1:1000
Mole Mass:49/37kDa
Location:
Concentration:
Sequence Similarity:Belongs to the peptidase C14A family. Contains 1 CARD domain.
Gene Id:842
SwissProt ID:P55211
Unigene:
Nucleotide Accession:
Tissue specificity:
Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Buffer condition:Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
Background:Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
Molar Function:
Western Blot:Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.