Anti-β-Catenin Mouse mAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:IgG1

Host:Mouse

Application:WB

Purify method:Affinity purified

Species:Human,Mouse,Rat

Gene Name:CTNNB1

Synonyms:EVR7; CTNNB; MRD19; armadillo;CTNNB1.

Gene Synonyms:Beta catenin ;Beta-catenin ;Cadherin associated protein ;Catenin (cadherin associated protein), beta 1, 88kDa ;Catenin beta 1 ;Catenin beta-1 ;CATNB ;CHBCAT ;CTNB1_HUMAN ;CTNNB ;CTNNB1 ;DKFZp686D02253 ;FLJ25606 ;FLJ37923 ;OTTHUMP00000162082 ;OTTHUMP00000165222 ;OTTHUMP00000165223 ;OTTHUMP00000209288 ;OTTHUMP00000209289.

Gene Full Name:

Gene Infomation:

Antigen:Purified recombinant human β-Catenin protein fragments expressed in E.coli.

Antigen Synonyms:

Clonality:Monclonal antibody

Source:

Reaction:β-Catenin Mouse mAb recognizes endogenous levels of total β-catenin protein.

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:5D6-D5-E9

Dilution:WB 1:1000

Mole Mass:92kDa

Location:Cytoplasm. Nucleus. Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell junction. Cell membrane. Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization.

Concentration:2.8mg/ml

Sequence Similarity:Belongs to the beta-catenin family.Contains 12 ARM repeats.

Gene Id:1499

SwissProt ID:P35222

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:PBS(pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.

Background:Key dowstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes.Involved in the regulation of cell adhesion. The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.