Anti-Collagen II Mouse mAb (7F7)
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Antibody type:Primary antibody
Label:Unconjugated
Modification:Unmodification
Isotype:IgG1
Host:Mouse
Application:IHC
Purify method:Affinity purified
Species:Human,Rat,Mouse
Gene Name:COL2A1
Synonyms:Alpha 1 type II collagen , AOM, Col2a1
Gene Synonyms:
Gene Full Name:
Gene Infomation:
Antigen:Synthetic peptide conjugated to KLH.
Antigen Synonyms:
Clonality:Monclonal antibody
Source:
Reaction:The Collagen II mouse monoclonal Antibody can detects endogenous Collagen II proteins.
Form:Liquid
Tested Applications:
- Western blot (1:100 to 1:500)
- Immunofluorescence (1:50 to 1:400)
- Immunohistochemistry (1:200 to 1:500)
- Flow cytometry analysis (1:200 to 1:500)
- Enzyme-linked Immunosorbent Assay (1:100-1:5000)
Clone:7F7-4B8-7H5
Dilution:IHC 1:200-500 (Optimal dilutions should be determined by the end user)
Mole Mass:142kDa
Location:Extracellular matrix, Secreted
Concentration:
Sequence Similarity:
Gene Id:1280
SwissProt ID:P02458
Unigene:
Nucleotide Accession:
Tissue specificity:
Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Buffer condition:PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
Background:Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces.SitesFeature keyPosition(s)Description
Molar Function:
Western Blot:Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.