Anti-Collagen IV Mouse(8E5) Mouse mAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:

Isotype:IgG

Host:Mouse

Application:IHC

Purify method:Affinity Chromatography

Species:Human,Mouse,Rat

Gene Name:COL4A1

Synonyms:Collagen alpha-1(IV) chain [Cleaved into: Arresten]

Gene Synonyms:BSVD; RATOR

Gene Full Name:

Gene Infomation:This gene encodes a type IV collagen alpha protein. Type IV collagen proteins are integral components of basement membranes. This gene shares a bidirectional promoter with a paralogous gene on the opposite strand. The protein consists of an amino-terminal 7S domain, a triple-helix forming collagenous domain, and a carboxy-terminal non-collagenous domain. It functions as part of a heterotrimer and interacts with other extracellular matrix components such as perlecans, proteoglycans, and laminins. In addition, proteolytic cleavage of the non-collagenous carboxy-terminal domain results in a biologically active fragment known as arresten, which has anti-angiogenic and tumor suppressor properties. Mutations in this gene cause porencephaly, cerebrovascular disease, and renal and muscular defects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

Antigen:Synthetic Peptide of Collagen IV

Antigen Synonyms:

Clonality:Monclonal antibody

Source:

Reaction:

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:IHC::1:50-300

Mole Mass:161

Location:Secreted > Extracellular space > Extracellular matrix > Basement membrane;

Concentration:

Sequence Similarity:Contains 1 collagen IV NC1 (C-terminal non-collagenous) domain.

Gene Id:1282

SwissProt ID:

Unigene:

Nucleotide Accession:

Tissue specificity:The antibody detects endogenous Collagen IV protein

Storage:

Buffer condition:PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Background:

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.