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Anti-Cytokeratin 7Rabbit pAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:Rabbit IgG

Host:Rabbit

Application:WB,IHC,ICC/IF,IP,FC

Purify method:Affinity Chromatography

Species:Human

Gene Name:

Synonyms:CK-7; CK7; cytokeratin 7; Cytokeratin-7; K2C7; K7; keratin 7; Keratin-7; KRT7; Sarcolectin; SCL;

Gene Synonyms:

Gene Full Name:

Gene Infomation:Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. K8 a type II cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins.

Antigen:A synthesized peptide derived from human Cytokeratin 7

Antigen Synonyms:

Clonality:Polyclonal antibody

Source:

Reaction:Cytokeratin 7 Antibodydetects endogenous levels of total Cytokeratin 7

Form:Liquid

Tested Applications:

  • Western blot (1:100 to 1:500)
  • Immunofluorescence (1:50 to 1:400)
  • Immunohistochemistry (1:200 to 1:500)
  • Flow cytometry analysis (1:200 to 1:500)
  • Enzyme-linked Immunosorbent Assay (1:100-1:5000)
NoteUsers are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:WB 1:5000~1:10000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:20

Mole Mass:51kDa

Location:Nucleus matrix;

Concentration:

Sequence Similarity:Belongs to the intermediate filament family.

Gene Id:

SwissProt ID:P08729

Unigene:

Nucleotide Accession:

Tissue specificity:Cytokeratin 7 Antibodydetects endogenous levels of total Cytokeratin 7

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Background:K7 a type II cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Phosphorylation of keratins at specific sites affects their organization, assembly dynamics, and their interaction with signaling molecules. Specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.