Anti-DDX3 Mouse mAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:IgG2a

Host:Mouse

Application:WB,IP,ICC

Purify method:Affinity purified

Species:Human,Rat,Mouse,Monkey

Gene Name:ddx3x

Synonyms:DBX;DDX3;HLP2;DDX14.

Gene Synonyms:ATP dependent RNA helicase DDX3X;ATP-dependent RNA helicase DDX3X;CAP Rf;DBX;DDX14;DDX3X;DDX3X_HUMAN;DEAD (Asp Glu Ala Asp) box polypeptide 3 X linked;DEAD box;DEAD box protein 3;DEAD box protein 3 X-chromosomal;DEAD box X isoform;DEAD/H (Asp Glu Ala Asp/

Gene Full Name:

Gene Infomation:

Antigen:Purified recombinant human DDX3 protein fragments expressed in E.coli.

Antigen Synonyms:

Clonality:Monclonal antibody

Source:Human

Reaction:This antibody detects endogenous levels of DDX3 and does not cross-react with related proteins.

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:6G8-F4-E3

Dilution:WB 1:1000,ICC 1:200

Mole Mass:75kDa

Location:Nucleus speckle.Cytoplasm.Mitochondrion outer membrane.Note: Located predominantly in nuclear speckles and,at low levels,throughout the cytoplasm.Located to the outer side of nuclear pore complexes (NPC).Shuttles between the nucleus and the cytoplasm in a

Concentration:

Sequence Similarity:Belongs to the DEAD box helicase family. DDX3/DED1 subfamily.Contains 1 helicase ATP-binding domain.Contains 1 helicase C-terminal domain.

Gene Id:1654

SwissProt ID:O00571

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide 0.1mg/mlBSA and 50% glycerol.

Background:

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.