Anti-DYKDDDDK-tag Mouse mAb

Antibody type:Tag antibody

Label:Unconjugated

Modification:Unmodification

Isotype:IgG1

Host:Mouse

Application:WB,IP

Purify method:Affinity purified

Species:Transfected

Gene Name:flag

Synonyms:FLAG

Gene Synonyms:FLAG

Gene Full Name:

Gene Infomation:

Antigen:DYKDDDDK synthetic peptide conjugated to KLH.

Antigen Synonyms:

Clonality:Monclonal antibody

Source:Artificial tag

Reaction:Transfected Only.

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:3A6-E5-C10

Dilution:WB 1:1000

Mole Mass:

Location:

Concentration:1.56mg/mL

Sequence Similarity:

Gene Id:

SwissProt ID:

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.

Background:Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.