Anti-E2F-1 Mouse mAb

100ug (NP10252) Please inquiry

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:IgG2b

Host:Mouse

Application:WB,IF,IP

Purify method:Affinity purified

Species:Human,Rat

Gene Name:e2f1

Synonyms:RBP3; E2F-1; RBAP1; RBBP3

Gene Synonyms:DmelCG6376 ; Dmel_CG6376 ; drosE2F1 ; E(Sev-CycE)3A ; E(var)3-93E ; E2-promoter binding facto ; E2F 1 ; E2F transcription factor 1 ; E2F-1 ; E2f-PA ; E2f-PB ; E2f-PC ; E2F1 ; E2f1 E2F transcription factor 1 ; E2F1_HUMAN ; Evar(3)164 ; KIAA4009 ; l(3)0717

Gene Full Name:

Gene Infomation:

Antigen:Purified recombinant human E2F-1 protein fragments expressed in E.coli.

Antigen Synonyms:

Clonality:Monclonal antibody

Source:Human

Reaction:This antibody detects endogenous levels of E2F-1 and does not cross-react with related proteins.

Form:Liquid

Tested Applications:

Western blot (1:100 to 1:500)
Immunofluorescence (1:50 to 1:400)
Immunohistochemistry (1:200 to 1:500)
Flow cytometry analysis (1:200 to 1:500)
Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:4G8-2C2-C12

Dilution:WB 1:500,ICC 1:100

Mole Mass:70kDa

Location:Nucleus

Concentration:5.79mg/mL

Sequence Similarity:Belongs to the E2F/DP family.

Gene Id:1869

SwissProt ID:Q01094

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.

Background:Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis.

Molar Function:

Western Blot:

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.