Anti-ERK1/2(Phospho-Thr202/Tyr204) Rabbit pAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:

Isotype:IgG

Host:Rabbit

Application:WB,IHC

Purify method:Affinity purified

Species:Human,Mouse,Rat

Gene Name:mapk3/mapk1

Synonyms:ERK 1;ERK 2;ERK-2;ERK1;ERK2;ERT1;ERT2;Extracellular signal regulated kinase 1;Extracellular signal regulated kinase 1;Extracellular signal regulated kinase 2;Extracellular signal regulated kinase 2;Extracellular signal-regulated kinase 2;HS44KDAP;HUMKER1A;Insulin stimulated MAP2 kinase;MAP kinase 1;MAP kinase 2;MAP kinase isoform p42;MAP kinase isoform p44;MAPK 1;MAPK 2;MAPK 3;MAPK 3;MAPK1;MAPK2;MAPK3;MGC20180;Microtubule associated protein 2 kinase;Mitogen activated protein kinase 1;Mitogen activated protein kinase 1;Mitogen activated protein kinase 2;Mitogen activated protein kinase 3;Mitogen activated protein kinase 3;Mitogen-activated protein kinase 1;Mitogen-activated protein kinase 2;MK01_HUMAN;p38;p40;p41;p41mapk;p42 MAPK;p42-MAPK;p42MAPK;p42MAPK;p44 ERK1;p44 MAPK;p44ERK1;p44ERK1;p44MAPK;p44MAPK;PRKM 1;PRKM 1;PRKM 2;PRKM 2;PRKM 3;PRKM 3;PRKM1;PRKM2;PRKM3;Protein kinase mitogen activated 1;Protein kinase mitogen activated 1;Protein kinase mitogen activated 2;Protein kinase mitogen activated 2;Protein kinase mitogen activated 3;Protein kinase mitogen activated 3;Protein tyrosine kinase ERK 2;Protein tyrosine kinase ERK 2.

Gene Synonyms:ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK/ERK; p38; p40; p41; ERK2; ERT1; MAPK2; PRKM1; PRKM2; P42MAPK; p41mapk

Gene Full Name:

Gene Infomation:The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq, Jul 2008]/The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. [provided by RefSeq, Jul 2008]

Antigen:The antiserum was produced against synthesized phosphopeptide derived from human ERK1/2 around the phosphorylation site of Thr202/Tyr204 (F-L-TP-E-YP-V-A).

Antigen Synonyms:

Clonality:Polyclonal antibody

Source:Human

Reaction:

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:

Mole Mass:42

Location:Cytoplasm. Nucleus. Note: Autophosphorylation at Thr-207 promotes nuclear localization./Cytoplasm › cytoskeleton › spindle. Nucleus. Cytoplasm › cytoskeleton › microtubule organizing center › centrosome. Cytoplasm. Note: Associated with the spindle during

Concentration:

Sequence Similarity:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.Contains 1 protein kinase domain./Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.Contains 1 protein kinase domain.

Gene Id:5595,5594

SwissProt ID:

Unigene:

Nucleotide Accession:

Tissue specificity:This antibody detects endogenous levels of ERK1 + ERK2 and does not cross-react with related proteins.

Storage:

Buffer condition:10mM PBS with 0.02% sodium azide, 50% glycerol.

Background:

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.