Anti-ERK5 Rabbit pAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:Rabbit IgG

Host:Rabbit

Application:WB,ICC/IF,IP,FC

Purify method:Affinity Chromatography

Species:Human,Mouse,Rat

Gene Name:

Synonyms:Big MAP kinase 1; BMK 1; BMK 1 kinase; BMK-1; BMK1; BMK1 Kinase; ERK 4; ERK 5; ERK-5; ERK4; ERK5; MAP kinase 7; MAPK 7; Mitogen Activated Protein Kinase;

Gene Synonyms:

Gene Full Name:

Gene Infomation:Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner (PubMed:20074560). Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual.

Antigen:A synthesized peptide derived from human ERK5

Antigen Synonyms:

Clonality:Polyclonal antibody

Source:

Reaction:ERK5 Antibody detects endogenous levels of ERK5

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:WB 1:1000~1:2000 ICC/IF 1:50~1:200 IP 1:50 FC 1:50

Mole Mass:115kDa

Location:Nucleus;

Concentration:

Sequence Similarity:Contains 1 nuclear receptor DNA-binding domain.

Gene Id:

SwissProt ID:Q13164

Unigene:

Nucleotide Accession:

Tissue specificity:ERK5 Antibody detects endogenous levels of ERK5

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Background:Erk5 (Mitogen-activated protein kinase 7, Big mitogen-activated protein kinase 1) is a member of the MAPK superfamily implicated in the regulation numerous cellular processes including proliferation, differentiation, and survival. In neuronal cells, Erk5 is required for NGF-induced neurite outgrowth, neuronal homeostasis, and survival. Erk5 is thought to play a role in blood vessel integrity via maintenance of endothelial cell migration and barrier function.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.