Anti-EpCAM Rabbit pAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:Rabbit IgG

Host:Rabbit

Application:WB,IHC

Purify method:Affinity Chromatography

Species:Human

Gene Name:

Synonyms:Epithelial cell adhesion molecule; Ep-CAM; CO17A; CD326; Cell surface glycoprotein Trop-1; EGP; Epithelial glycoprotein 314; EGP314; hEGP314; KS 1/4 antigen; KSA;

Gene Synonyms:

Gene Full Name:

Gene Infomation:ATF5 or Activating transcription factor 5, binds to cAMP inducible promoters and is involved in gene transcription. This protein binds the cAMP response element (CRE) (consensus: 5-GTGACGT[AC][AG]-3), a sequence present in many viral and cellular promoters. ATF5 plays a role in inhibition of nerve growth factor induced neuronal outgrowth and regulation of neurogenesis.

Antigen:A synthesized peptide derived from human EpCAM

Antigen Synonyms:

Clonality:Polyclonal antibody

Source:

Reaction:EpCAM Antibody detects endogenous levels of EpCAM

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:

Dilution:WB 1:1000~1:2000 IHC 1:50~1:200

Mole Mass:35kDa

Location:Cytoplasm > Cytoskeleton > Microtubule organizing center > Centrosome;

Concentration:

Sequence Similarity:Belongs to the bZIP family.

Gene Id:

SwissProt ID:P16422

Unigene:

Nucleotide Accession:

Tissue specificity:EpCAM Antibody detects endogenous levels of EpCAM

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Background:Epithelial cell adhesion and activating molecule (EpCAM/CD326) is a transmembrane glycoprotein that mediates Ca2+ independent, homophilic adhesions on the basolateral surface of most epithelial cells. The antigen is being used as a target for immunotherapy treatment of human carcinomas.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.