Anti-FAK Mouse mAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:IgG1

Host:Mouse

Application:WB,IF,IP

Purify method:Affinity purified

Species:Human

Gene Name:ptk2

Synonyms:FAK; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK.

Gene Synonyms:FADK 1;FADK;FAK 1;FAK 1;FAK related non kinase polypeptide;FAK1;FAK1_HUMAN;Focal adhesion kinase 1;Focal adhesion kinase 1;Focal adhesion Kinase;Focal adhesion kinase isoform FAK Del33;Focal adhesion kinase related nonkinase;FRNK;p125FAK;pp125FAK;pp125FAK

Gene Full Name:

Gene Infomation:

Antigen:Purified recombinant human FAK protein fragments expressed in E.coli.

Antigen Synonyms:

Clonality:Monclonal antibody

Source:Human

Reaction:This antibody detects endogenous levels of FAK and does not cross-react with related proteins.

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:3D8-H11-E12

Dilution:WB 1:1000,ICC 1:200

Mole Mass:125kDa

Location:Cell junction › focal adhesion;_x000D_Cell membrane; Peripheral membrane protein; Cytoplasmic side;_x000D_Cytoplasm › cell cortex;_x000D_Cytoplasm › cytoskeleton;_x000D_Cytoplasm › cytoskeleton › microtubule organizing center › centrosome;_x000D_Nucleus

Concentration:

Sequence Similarity:Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily. Cotains 1 FERM domain. Contains 1 protein kinase domain.

Gene Id:5747

SwissProt ID:Q05397

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:PBS(pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.

Background:Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.