Anti-FAS(C-term) Mouse mAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:IgG1

Host:Mouse

Application:WB

Purify method:Affinity purified

Species:Human

Gene Name:fas

Synonyms:APT1; CD95; FAS1; APO-1; FASTM; ALPS1A; TNFRSF6

Gene Synonyms:ALPS 1A;ALPS1A;APO 1;Apo 1 antigen;APO 1 cell surface antigen;Apo-1 antigen;APO1;Apo1 antigen;APO1 cell surface antigen;Apoptosis antigen 1;Apoptosis mediating surface antigen FAS;Apoptosis-mediating surface antigen FAS;APT 1;APT1;CD 95;CD 95 antigen; CD9

Gene Full Name:

Gene Infomation:

Antigen:Purified recombinant human FAS (C-terminus) protein fragments expressed in E.coli.

Antigen Synonyms:

Clonality:Monclonal antibody

Source:Human

Reaction:This antibody detects endogenous levels of FAS (C-terminus) and does not cross-react with related proteins.

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:4C3-G7-B7-H5

Dilution:WB 1:1000

Mole Mass:45kDa

Location:Isoform 1: Cell membrane; Single-pass type I membrane protein _x000D_Isoform 2: Secreted _x000D_Isoform 3: Secreted _x000D_Isoform 4: Secreted_x000D_Isoform 5: Secreted _x000D_Isoform 6: Secreted

Concentration:

Sequence Similarity:Contains 1 death domain._x000D_Contains 3 TNFR-Cys repeats

Gene Id:355

SwissProt ID:P25445

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4 150 mM NaCl) with 0.02% sodium azide 50% glycerol

Background:Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.