Anti-Fyn Mouse mAb

Antibody type:Primary antibody

Label:Unconjugated

Modification:Unmodification

Isotype:IgG2b

Host:Mouse

Application:WB,ICC

Purify method:Affinity purified

Species:Human,Monkey,Rat,Mouse

Gene Name:fyn

Synonyms:SLK;SYN;p59-FYN.

Gene Synonyms:C syn protooncogene;Fyn;FYN oncogene related to SRC FGR YES;FYN_HUMAN;MGC45350;OKT3 induced calcium influx regulator;P59 FYN;p59-Fyn;Protein tyrosine kinase fyn;Proto oncogene tyrosine protein kinase fyn;Proto-oncogene c-Fyn;Proto-oncogene Syn;Protooncoge

Gene Full Name:

Gene Infomation:

Antigen:Purified recombinant human Fyn protein fragments expressed in E.coli.

Antigen Synonyms:

Clonality:Monclonal antibody

Source:Human

Reaction:This antibody detects endogenous levels of Fyn and does not cross-react with related proteins.

Form:Liquid

Tested Applications:

• Western blot (1:100 to 1:500)
• Immunofluorescence (1:50 to 1:400)
• Immunohistochemistry (1:200 to 1:500)
• Flow cytometry analysis (1:200 to 1:500)
• Enzyme-linked Immunosorbent Assay (1:100-1:5000)

Note: Users are strongly advised to determine the optimal dilution of antibody to use for their specific applications.

Clone:4B8-E7-A8

Dilution:WB 1:500,ICC 1:50

Mole Mass:59kDa

Location:Cytoplasm.Nucleus.Cell membrane.Note: Present and active in lipid rafts.Palmitoylation is crucial for proper trafficking.

Concentration:

Sequence Similarity:Belongs to the protein kinase superfamily.Tyr protein kinase family.SRC subfamily.Contains 1 protein kinase domain.Contains 1 SH2 domain.Contains 1 SH3 domain.

Gene Id:2534

SwissProt ID:P06241

Unigene:

Nucleotide Accession:

Tissue specificity:

Storage:Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

Buffer condition:Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.

Background:Non-receptor tyrosine-protein kinase that plays a role in many biological processes including regulation of cell growth and survival,cell adhesion,integrin-mediated signaling,cytoskeletal remodeling,cell motility,immune response and axon guidance.Inactive FYN is phosphorylated on its C-terminal tail within the catalytic domain.Following activation by PKA,the protein subsequently associates with PTK2/FAK1,allowing PTK2/FAK1 phosphorylation,activation and targeting to focal adhesions.Involved in the regulation of cell adhesion and motility through phosphorylation of CTNNB1 (beta-catenin) and CTNND1 (delta-catenin). Regulates cytoskeletal remodeling by phosphorylating several proteins including the actin regulator WAS and the microtubule-associated proteins MAP2 and MAPT.Promotes cell survival by phosphorylating AGAP2/PIKE-A and preventing its apoptotic cleavage.Participates in signal transduction pathways that regulate the integrity of the glomerular slit diaphragm (an essential part of the glomerular filter of the kidney) by phosphorylating several slit diaphragm components including NPHS1,KIRREL and TRPC6.Plays a role in neural processes by phosphorylating DPYSL2,a multifunctional adapter protein within the central nervous system,ARHGAP32,a regulator for Rho family GTPases implicated in various neural functions, and SNCA,a small pre-synaptic protein.Participates in the downstream signaling pathways that lead to T-cell differentiation and proliferation following T-cell receptor (TCR) stimulation.Also participates in negative feedback regulation of TCR signaling through phosphorylation of PAG1,thereby promoting interaction between PAG1 and CSK and recruitment of CSK to lipid rafts.CSK maintains LCK and FYN in an inactive form.Promotes CD28-induced phosphorylation of VAV1.

Molar Function:

Western Blot: 

Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Storage: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Notes: For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.